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Proteintech trap1
Trap1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+trap1/CAT-1+Antibody/pmc12906242-77-17-18
Average 93 stars, based on 51 article reviews

trap1 - by Bioz Stars, 2026-10

93/100 stars

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MTT Assay:

Article Title: Stress granules affect the dual PI3K/mTOR inhibitor response by regulating the mitochondrial unfolded protein response
Article Snippet: SKOV3 and A2780 ovarian cancer cells were grown in RPMI-1640 (Gibco Life Technologies, USA) supplemented with 10% fetal bovine serum (Invitrogen, USA) at 37 °C in a 5% CO2 concentration. .. Reagents used in this study include the following: Methylthiazolyldiphenyl-tetrazolium bromide (MTT)(S6821), PKI-402(S2739), cycloheximide (CHX)(NSC-185) and Thapsigargin (Tg) (S7895)were commercially sourced from Selleckchem (Houston, TX, USA), anti-G3BP1 (13057-2-AP), anti-eIF2α (11233-1-AP), anti-4EBP1 (60246-1-Ig), anti-caspase-9(66169-1-Ig), anti-Bcl-2 (26593-1-AP), anti-Bax (50599-2-Ig), anti-Hsp60 (15282-1-AP), anti-Lonp (66043-1-Ig), anti-Clpp (15698-1-AP), anti-Trap1 (10325-1-AP), anti-Actin(HRP-60008), anti-VDAC (10866-1-AP) (Proteintech, Chicago, IL, USA), anti-YB-1(sc-101198), anti-ATF5(sc-377168), anti-p-Akt(Ser473) (sc-101629)(Santa Cruz, CA, USA), p- eIF2α(Ser51)(3398), p-4EBP1(Thr37/46)(2855), p-Akt(Thr308) (13038), p-P70S6K(9204) (Cell Signaling Technology, USA). ..

Article Title: Stress granules affect the dual PI3K/mTOR inhibitor response by regulating the mitochondrial unfolded protein response
Article Snippet: .. Reagents and antibodies Reagents used in this study include the following: 3- (4-dimethylthiazol-2-yl) -2,5-di-phenyltetrazolium bromide (MTT), PKI-402, cycloheximide (CHX) and Thapsigargin (Tg) were commercially sourced from Selleckchem (Houston, TX, USA), anti-G3BP1, anti-eIF2α, anti-4EBP1, anti-caspase-9, anti-Bcl-2, anti-Bax, anti-Hsp60, anti-Lonp, anti-Clpp, anti-Trap1, anti-Actin, anti-vdac (Proteintech, Chicago, IL, USA), anti-YB-1, anti-ATF5, anti-Akt,anti-p-Akt(Ser473) (Santa Cruz, CA, USA), p- eIF2α(Ser51), p-4EBP1(Thr46)( Cell Signaling Technology, USA). ..

Article Title: Stress granules affect the dual PI3K/mTOR inhibitor response by regulating the mitochondrial unfolded protein response.
Article Snippet: SKOV3 and A2780 ovarian cancer cells were grown in RPMI-1640 (Gibco Life Technologies, USA) supplemented with 10% fetal bovine serum (Invitrogen, USA) at 37 °C in a 5% CO2 concentration. .. Reagents used in this study include the following: Methylthiazolyldiphenyl-tetrazolium bromide (MTT)(S6821), PKI-402(S2739), cycloheximide (CHX)(NSC-185) and Thapsigargin (Tg) (S7895)were commercially sourced from Selleckchem (Houston, TX, USA), anti-G3BP1 (13057-2-AP), anti-eIF2α (11233-1-AP), anti-4EBP1 (60246-1-Ig), anti-caspase-9(66169-1-Ig), anti-Bcl-2 (26593-1-AP), anti-Bax (50599-2-Ig), anti-Hsp60 (15282- 1-AP), anti-Lonp (66043-1-Ig), anti-Clpp (15698-1- AP), anti-Trap1 (10325-1-AP), anti-Actin(HRP-60008), anti-VDAC (10866-1-AP) (Proteintech, Chicago, IL, USA), anti-YB-1(sc-101198), anti-ATF5(sc-377168), anti-p-Akt(Ser473) (sc-101629)(Santa Cruz, CA, USA), p- eIF2α(Ser51)(3398), p-4EBP1(Thr37/46)(2855), p-Akt(Thr308) (13038), p-P70S6K(9204) (Cell Signaling Technology, USA). ..

Article Title: Stress granules affect the dual PI3K/mTOR inhibitor response by regulating the mitochondrial unfolded protein response.
Article Snippet: Methods and materials Cell lines and cell culture SKOV3 and A2780 ovarian cancer cells were grown in RPMI-1640 (Gibco Life Technologies, USA) supplemented with 10% fetal bovine serum (Invitrogen, USA) at 37 °C in a 5% CO2 concentration. .. Reagents and antibodies Reagents used in this study include the following: Methylthiazolyldiphenyl-tetrazolium bromide (MTT)(S6821), PKI-402(S2739), cycloheximide (CHX)(NSC-185) and Thapsigargin (Tg) (S7895)were commercially sourced from Selleckchem (Houston, TX, USA), anti-G3BP1 (13057-2-AP), anti-eIF2α (11233-1-AP), anti-4EBP1 (60246-1-Ig), anti-caspase-9(66169-1-Ig), anti-Bcl-2 (26593-1-AP), anti-Bax (50599-2-Ig), anti-Hsp60 (152821-AP), anti-Lonp (66043-1-Ig), anti-Clpp (15698-1AP), anti-Trap1 (10325-1-AP), anti-Actin(HRP-60008), anti-VDAC (10866-1-AP) (Proteintech, Chicago, IL, USA), anti-YB-1(sc-101198), anti-ATF5(sc-377168), anti-p-Akt(Ser473) (sc-101629)(Santa Cruz, CA, USA), peIF2α(Ser51)(3398), p-4EBP1(Thr37/46)(2855), p-Akt(Thr308) (13038), p-P70S6K(9204) (Cell Signaling Technology, USA). ..

Immunoprecipitation:

Article Title: An orally administered gene editing nanoparticle boosts chemo-immunotherapy in colorectal cancer.
Article Snippet: Chemoresistance and immunosuppression are common obstacles to the efficacy of chemo-immunotherapy in colorectal cancer (CRC) and are regulated by mitochondrial chaperone proteins.. Here we show that the disruption of the tumour necrosis factor receptor-associated protein 1 (TRAP1) gene, which encodes a mitochondrial chaperone in tumour cells, causes the translocation of cyclophilin D in tumour cells.. This process results in the continuous opening of the mitochondrial permeability transition pore, which enhances chemotherapy-induced cell necrosis and promotes immune responses.

Western Blot:

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$a Sequence alignment of the C-terminal regions of human FATE1 and Mff generated with Jalview using the T-Coffee algorithm under default settings. Amino acid numbering corresponds to Mff. b Coiled coil domain prediction of FATE1 using the COILS program. Mff and Bnip3 were included as positive and negative controls, respectively. Predicted probabilities for coiled coil formation are shown for scanning windows of 14, 21 and 28 residues. c Schematic comparison of the domain architecture of Mff and FATE1, highlighting sequence homologies, including the predicted coiled coil domain and transmembrane region. R1 and R2 indicate the short repeat motifs in Mff involved in Drp1 recruitment. d Representative widefield images of GFP-Ctrl, GFP-FATE1, or GFP-Mff co-expressed with RFP-KDEL (ER marker) for 24 h in HeLa cells. Blue arrows, ER-localized FATE1 at perinuclear regions. e Representative widefield images of GFP-Ctrl, GFP-FATE1, or GFP-Mff co-expressed with mito-RFP (mitochondrial marker) for 24 h in HeLa cells. f Subcellular fractionation of HeLa cells transfected with GFP-tagged constructs as indicated. Fractionation yielded cytosolic (S1), rest-mitochondrial (P1) and purified mitochondrial (P2) fractions, analyzed by immunoblotting. g Representative widefield images of MCF-7 cells expressing GFP-FATE1 or GFP-Mff for 24 h and IF stained for mitochondrial matrix protein TRAP1. Plot profiles along yellow lines in zoom images show fluorescence intensity of GFP and TRAP1 signals. h STED super-resolution microscopy of HeLa cells expressing pcDNA-FATE1 and IF stained for FATE1 in combination with Cox IV (IMM marker) or Tom20 (OMM marker). Plot profiles show fluorescence intensity of FATE1 and Cox IV or Tom20. Scale bars, 10 µm$

Journal: bioRxiv

Article Title: The Cancer/Testis Antigen FATE1 Antagonizes Fission and Preserves Mitochondrial Network Integrity under Cytotoxic Stress

doi: 10.1101/2025.08.18.670752

Figure Lengend Snippet: a Sequence alignment of the C-terminal regions of human FATE1 and Mff generated with Jalview using the T-Coffee algorithm under default settings. Amino acid numbering corresponds to Mff. b Coiled coil domain prediction of FATE1 using the COILS program. Mff and Bnip3 were included as positive and negative controls, respectively. Predicted probabilities for coiled coil formation are shown for scanning windows of 14, 21 and 28 residues. c Schematic comparison of the domain architecture of Mff and FATE1, highlighting sequence homologies, including the predicted coiled coil domain and transmembrane region. R1 and R2 indicate the short repeat motifs in Mff involved in Drp1 recruitment. d Representative widefield images of GFP-Ctrl, GFP-FATE1, or GFP-Mff co-expressed with RFP-KDEL (ER marker) for 24 h in HeLa cells. Blue arrows, ER-localized FATE1 at perinuclear regions. e Representative widefield images of GFP-Ctrl, GFP-FATE1, or GFP-Mff co-expressed with mito-RFP (mitochondrial marker) for 24 h in HeLa cells. f Subcellular fractionation of HeLa cells transfected with GFP-tagged constructs as indicated. Fractionation yielded cytosolic (S1), rest-mitochondrial (P1) and purified mitochondrial (P2) fractions, analyzed by immunoblotting. g Representative widefield images of MCF-7 cells expressing GFP-FATE1 or GFP-Mff for 24 h and IF stained for mitochondrial matrix protein TRAP1. Plot profiles along yellow lines in zoom images show fluorescence intensity of GFP and TRAP1 signals. h STED super-resolution microscopy of HeLa cells expressing pcDNA-FATE1 and IF stained for FATE1 in combination with Cox IV (IMM marker) or Tom20 (OMM marker). Plot profiles show fluorescence intensity of FATE1 and Cox IV or Tom20. Scale bars, 10 µm

Article Snippet: Cells were then incubated with primary antibodies against Tom20 (Santa Cruz, no. sc-11415; 1:200) or FATE1 (Santa Cruz Biotechnology, no. sc-101220; 1:100) for 1 h at room temperature, or against COX IV (Cell Signaling, no. 4850S; 1:50) or TRAP1 (Novus Biologicals, no. NB300-555; 1:100) antibodies at 4°C overnight.

Techniques: Sequencing, Generated, Comparison, Marker, Fractionation, Transfection, Construct, Purification, Western Blot, Expressing, Staining, Fluorescence, Super-Resolution Microscopy

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